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third-generation lentiviral particles  (Applied Biological Materials Inc)

 
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    Applied Biological Materials Inc third-generation lentiviral particles
    Exo70 overexpression reinforces NMDAR synaptic availability upon mTBI induction. Samples from the dorsal hippocampus were analyzed by Western blot. Here, 30 µg of protein samples were resolved in 10% SDS-PAGE and transferred to PVDF membranes. Tubulin and Actin were used as loading controls. ( A ) Characterization of the <t>lentiviral</t> expression in all experimental groups using GFP and HA antibodies. ( B ) Densitometric analysis of HA-Exo70. ( C ) Western blot analyzing NMDAR synaptic localization after mTBI in injected mice. Samples were analyzed using GluN2B p1472, GluN2B p1336, and total GluN2B antibodies. ( D – F ) Densitometric analysis normalized with loading controls before normalization with Sham-GFP control samples. ( G ) Western blot analyzing NMDAR synaptic-signaling-related proteins after mTBI induction in Exo70-overexpressing samples. Samples were analyzed using pCREB and pERK1/2 antibodies. ( H , I ) The graphs show the densitometric analysis normalized with loading controls before normalization with Sham-GFP control samples. White circles indicate individual experiments. Values represent means ± SEM, n = 5 mice per experimental group. Statistical differences were calculated by two-way ANOVA, followed by post hoc Bonferroni’s test. * p < 0.05, ** p < 0.01, *** p < 0.001, ns: statistically non-significant difference.
    Third Generation Lentiviral Particles, supplied by Applied Biological Materials Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/third-generation lentiviral particles/product/Applied Biological Materials Inc
    Average 90 stars, based on 1 article reviews
    third-generation lentiviral particles - by Bioz Stars, 2026-03
    90/100 stars

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    1) Product Images from "Exo70 Protects Against Memory and Synaptic Impairments Following Mild Traumatic Brain Injury"

    Article Title: Exo70 Protects Against Memory and Synaptic Impairments Following Mild Traumatic Brain Injury

    Journal: Antioxidants

    doi: 10.3390/antiox14060640

    Exo70 overexpression reinforces NMDAR synaptic availability upon mTBI induction. Samples from the dorsal hippocampus were analyzed by Western blot. Here, 30 µg of protein samples were resolved in 10% SDS-PAGE and transferred to PVDF membranes. Tubulin and Actin were used as loading controls. ( A ) Characterization of the lentiviral expression in all experimental groups using GFP and HA antibodies. ( B ) Densitometric analysis of HA-Exo70. ( C ) Western blot analyzing NMDAR synaptic localization after mTBI in injected mice. Samples were analyzed using GluN2B p1472, GluN2B p1336, and total GluN2B antibodies. ( D – F ) Densitometric analysis normalized with loading controls before normalization with Sham-GFP control samples. ( G ) Western blot analyzing NMDAR synaptic-signaling-related proteins after mTBI induction in Exo70-overexpressing samples. Samples were analyzed using pCREB and pERK1/2 antibodies. ( H , I ) The graphs show the densitometric analysis normalized with loading controls before normalization with Sham-GFP control samples. White circles indicate individual experiments. Values represent means ± SEM, n = 5 mice per experimental group. Statistical differences were calculated by two-way ANOVA, followed by post hoc Bonferroni’s test. * p < 0.05, ** p < 0.01, *** p < 0.001, ns: statistically non-significant difference.
    Figure Legend Snippet: Exo70 overexpression reinforces NMDAR synaptic availability upon mTBI induction. Samples from the dorsal hippocampus were analyzed by Western blot. Here, 30 µg of protein samples were resolved in 10% SDS-PAGE and transferred to PVDF membranes. Tubulin and Actin were used as loading controls. ( A ) Characterization of the lentiviral expression in all experimental groups using GFP and HA antibodies. ( B ) Densitometric analysis of HA-Exo70. ( C ) Western blot analyzing NMDAR synaptic localization after mTBI in injected mice. Samples were analyzed using GluN2B p1472, GluN2B p1336, and total GluN2B antibodies. ( D – F ) Densitometric analysis normalized with loading controls before normalization with Sham-GFP control samples. ( G ) Western blot analyzing NMDAR synaptic-signaling-related proteins after mTBI induction in Exo70-overexpressing samples. Samples were analyzed using pCREB and pERK1/2 antibodies. ( H , I ) The graphs show the densitometric analysis normalized with loading controls before normalization with Sham-GFP control samples. White circles indicate individual experiments. Values represent means ± SEM, n = 5 mice per experimental group. Statistical differences were calculated by two-way ANOVA, followed by post hoc Bonferroni’s test. * p < 0.05, ** p < 0.01, *** p < 0.001, ns: statistically non-significant difference.

    Techniques Used: Over Expression, Western Blot, SDS Page, Expressing, Injection, Control



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    Image Search Results


    Exo70 overexpression reinforces NMDAR synaptic availability upon mTBI induction. Samples from the dorsal hippocampus were analyzed by Western blot. Here, 30 µg of protein samples were resolved in 10% SDS-PAGE and transferred to PVDF membranes. Tubulin and Actin were used as loading controls. ( A ) Characterization of the lentiviral expression in all experimental groups using GFP and HA antibodies. ( B ) Densitometric analysis of HA-Exo70. ( C ) Western blot analyzing NMDAR synaptic localization after mTBI in injected mice. Samples were analyzed using GluN2B p1472, GluN2B p1336, and total GluN2B antibodies. ( D – F ) Densitometric analysis normalized with loading controls before normalization with Sham-GFP control samples. ( G ) Western blot analyzing NMDAR synaptic-signaling-related proteins after mTBI induction in Exo70-overexpressing samples. Samples were analyzed using pCREB and pERK1/2 antibodies. ( H , I ) The graphs show the densitometric analysis normalized with loading controls before normalization with Sham-GFP control samples. White circles indicate individual experiments. Values represent means ± SEM, n = 5 mice per experimental group. Statistical differences were calculated by two-way ANOVA, followed by post hoc Bonferroni’s test. * p < 0.05, ** p < 0.01, *** p < 0.001, ns: statistically non-significant difference.

    Journal: Antioxidants

    Article Title: Exo70 Protects Against Memory and Synaptic Impairments Following Mild Traumatic Brain Injury

    doi: 10.3390/antiox14060640

    Figure Lengend Snippet: Exo70 overexpression reinforces NMDAR synaptic availability upon mTBI induction. Samples from the dorsal hippocampus were analyzed by Western blot. Here, 30 µg of protein samples were resolved in 10% SDS-PAGE and transferred to PVDF membranes. Tubulin and Actin were used as loading controls. ( A ) Characterization of the lentiviral expression in all experimental groups using GFP and HA antibodies. ( B ) Densitometric analysis of HA-Exo70. ( C ) Western blot analyzing NMDAR synaptic localization after mTBI in injected mice. Samples were analyzed using GluN2B p1472, GluN2B p1336, and total GluN2B antibodies. ( D – F ) Densitometric analysis normalized with loading controls before normalization with Sham-GFP control samples. ( G ) Western blot analyzing NMDAR synaptic-signaling-related proteins after mTBI induction in Exo70-overexpressing samples. Samples were analyzed using pCREB and pERK1/2 antibodies. ( H , I ) The graphs show the densitometric analysis normalized with loading controls before normalization with Sham-GFP control samples. White circles indicate individual experiments. Values represent means ± SEM, n = 5 mice per experimental group. Statistical differences were calculated by two-way ANOVA, followed by post hoc Bonferroni’s test. * p < 0.05, ** p < 0.01, *** p < 0.001, ns: statistically non-significant difference.

    Article Snippet: Third-generation lentiviral particles were packaged by and acquired from Applied Biological Materials (ABM, Canada) based on the lentiviral vector FUG-1D2A-Exo70-W [ ].

    Techniques: Over Expression, Western Blot, SDS Page, Expressing, Injection, Control